Product Name :Osteopontin polyclonal antibody
Reactivity :Human, Mouse, Rat
Applications :WB IHC IF ELISA
Application_all :WB:1:500-1:2000
IHC:1:100-1:500
IF:1:100-1:500
ELISA:1:5,000-1:10,000
Background :Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
Product :0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :Osteopontin polyclonal antibody detects endogenous levels of Osteopontin protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :SPP-1, SPP1, BNSP, OPN, PSEC0156, BNSP, Bone sialoprotein 1, BSP I, BSPI, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, OPN, Osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, OSTP, PSEC0156, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), Secreted phosphoprotein 1, SPP 1, SPP-1, SPP1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin,
Immunogen :KLH conjugated synthetic peptide derived from mouse OPN: 221-280/294
