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Histone H4 (sDi-Methyl R3) polyclonal antibody

货号: BS8455

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS8455

Product Name :Histone H4 (sDi-Methyl R3) polyclonal antibody

Swiss-Prot :P62805

Host :Rabbit

Reactivity :Human,Mouse,Rat,Other (Wide Range)

Applications :WB IHC IF

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Background :Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Histone H4 (sDi-Methyl R3) polyclonal antibody detects endogenous levels of histone H4 only when Symmetric Di-Methyled at Arg3.

BiowMW :~ 11 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Examples of Crosstalk Between Post-translational Modifications,

Alternative Name :H4R3me2s

Immunogen :Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H4, sDi-methylated at R3.

Conjugate :Unconjugated

Modification :Methylated

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Histone H4 (sDi-Methyl R3) antibody.

Dot-blot analysis of all sorts of methylation peptides using Histone H4 (sDi-Methyl R3) antibody.

Immunofluorescence analysis of 293T cell using Histone H4 (sDi-Methyl R3) antibody. Blue: DAPI for nuclear staining.