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14-3-3 θ/YWHAQ (phospho-S232) polyclonal antibody

货号: BS64156

价格：￥ ——

货期：现货（1-2个工作日）

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No. :BS64156

Product Name :14-3-3 θ/YWHAQ (phospho-S232) polyclonal antibody

Swiss-Prot :P27348

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-YWHAQ (S232) polyclonal antibody detects endogenous levels of YWHAQ protein only when phosphorylated at Ser232.

BiowMW :~ 28 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQ

Immunogen :Synthetic phosphopeptide derived from human YWHAQ around the phosphorylation site of Serine 232.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-YWHAQ (S232) polyclonal antibody at 1:500 dillution Lane1: Hela whole cell lysate Lane2: Hela treated with LPS(100ng/ml, 30min) whole cell lysate Lane3: C6 whole cell lysate Lane4: C6 treated with EGF(0.1ng/ml, 30min) whole cell lysate Lane5: BV2 whole cell lysate Lane6: BV2 treated with LPS(100ng/ml, 30min) whole cell lysate