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EIF4G1 (phospho-S1148) polyclonal antibody

货号: BS64046

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64046

Product Name :EIF4G1 (phospho-S1148) polyclonal antibody

Swiss-Prot :Q04637

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex .

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-EIF4G1(S1148) pAbdetects endogenous levels of EIF4G1 protein around the phosphorylation site of S1148.

BiowMW :~ 175 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Dopamine Signaling in Parkinson's Disease Plathway,

Alternative Name :Eukaryotic translation initiation factor 4 gamma 1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220; EIF4G1; EIF4F; EIF4G; EIF4GI

Immunogen :A synthetic peptide corresponding to residues in Human EIF4G1 around the phosphorylation site of S1148.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-EIF4G1(S1148) polyclonal antibody at 1:500 dilution Lane1:THP-1 treated with EGF(0.1ng/m) 30 min whole cell lysate Lane2:PC12 treated with EGF(0.1ng/m) 30 min whole cell lysate Lane3:Raw264.7 treated with EGF(0.1ng/m) 30 min whole cell lysate