产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

APAF1 polyclonal antibody

货号: BS60866

价格：￥ ——

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS60866

Product Name :APAF1 polyclonal antibody

Swiss-Prot :O14727

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFκB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKγ. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFκB in response to bacterial lipopoly-saccharides.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :APAF1 polyclonal antibody detects endogenous levels of APAF1 protein.

BiowMW :~ 135 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Mitochondrial Control of Apoptosis,

Alternative Name :Apoptotic protease-activating factor 1; APAF-1; APAF1; KIAA0413

Immunogen :A synthetic peptide corresponding to residues in Human APAF1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of APAF1 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of APAF1 pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasm staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Design, synthesis and in vitro evaluation of novel ursolic acid derivatives as potential anticancer agents

JournalEuropean Journal Of Medicinal Chemistry

ApplicationWB

IF6.514

SpeciesHuman

PMID25841199
Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

JournalParasitology

ApplicationWB

IF3.234

SpeciesHuman

PMID28073393