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Histone H2A.X (phospho-S139) polyclonal antibody

货号: BS4760

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4760

Product Name :Histone H2A.X (phospho-S139) polyclonal antibody

Swiss-Prot :P16104

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Histone H2A.X (S139) polyclonal antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.

BiowMW :~ 15 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Histone Lysine Methylation Pathway,

Alternative Name :Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Immunogen :Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Histone H2A.X (S139) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with colchicine(0.2µg/ML,24h) Lane2:sp2/0 cell lysate treated with colchicine(0.2µg/ML,24h) Lane3:PC12 cell lysate treated with colchicine(0.2µg/ML,24h)

Immunohistochemistry (IHC) analyzes of p-Histone H2A.X (S139) pAb in paraffin-embedded human brain tissue.

Ribosomal protein L6 (RPL6) is recruited to DNA damage sites in a poly(ADP-ribose) polymerase–dependent manner and regulates the DNA damage response

JournalJournal Of Biological Chemistry

ApplicationWB,IF

IF4.106

SpeciesHuman

PMID30598506
Cruciform DNA in mouse growing oocytes: Its dynamics and its relationship with DNA transcription

JournalPLoS One

ApplicationIF

IF3.24

SpeciesMouse

PMID33079963
ATM–ESCO2–SMC3 axis promotes 53BP1 recruitment in response to DNA damage and safeguards genome integrity by stabilizing cohesin complex

JournalNUCLEIC ACIDS RESEARCH

ApplicationIF

IF14.9

SpeciesHuman

PMID37377435