产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Histone H2A.X (phospho-S139) polyclonal antibody

货号: BS4760

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4760

Product Name :Histone H2A.X (phospho-S139) polyclonal antibody

Swiss-Prot :P16104

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Histone H2A.X (S139) polyclonal antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.

BiowMW :~ 15 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Histone Lysine Methylation Pathway,

Alternative Name :Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Immunogen :Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Histone H2A.X (S139) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with colchicine(0.2µg/ML,24h) Lane2:sp2/0 cell lysate treated with colchicine(0.2µg/ML,24h) Lane3:PC12 cell lysate treated with colchicine(0.2µg/ML,24h)

Immunohistochemistry (IHC) analyzes of p-Histone H2A.X (S139) pAb in paraffin-embedded human brain tissue.

The effects of DNA double-strand breaks on mouse oocyte meiotic maturation

JournalCell Cycle

ApplicationIF

IF4.534

SpeciesMouse

PMID23518501
Attenuated LKB1-SIK1 signaling promotes epithelial-mesenchymal transition and radioresistance of non–small cell lung cancer cells

JournalChinese Journal of Cancer

ApplicationWB

IF3.62

SpeciesHuman

PMID27266881
Sulforaphane induces p53‑deficient SW480 cell apoptosis via the ROS‑MAPK signaling pathway

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID28944886
Inhibition of ribosomal RNA processing 15 Homolog (RRP15), which is overexpressed in hepatocellular carcinoma, suppresses tumour growth via induction of senescence and apoptosis

JournalCancer Letters

ApplicationIF,WB

IF8.679

SpeciesHuman

PMID34343634
Mycotoxin zearalenone exposure impairs genomic stability of swine follicular granulosa cells in vitro

JournalInternational Journal of Biological Sciences

ApplicationIF

IF4.858

SpeciesPig

PMID29559847