产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

FAK (phospho-Y397) polyclonal antibody

货号: BS4617

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4617

Product Name :FAK (phospho-Y397) polyclonal antibody

Swiss-Prot :Q05397

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FAK (phospho-Y397) polyclonal antibody detects endogenous levels of FAK protein only when phosphorylated at Tyr397.

BiowMW :~ 125 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Tumor Angiogenesis,Contribution of ECM and Cytoskeletal Factors to EMT,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1

Immunogen :Synthetic phosphopeptide derived from human FAK around the phosphorylation site of Serine 397.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of FAK (phospho-Y397) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-FAK (Y397) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-

JournalBreast Cancer Research

ApplicationWB

IF5.676

SpeciesHuman

PMID25990368
Herbal compound Teng‑Long‑Bu‑Zhong‑Tang inhibits metastasis in human RKO colon carcinoma

JournalOncology Letters

ApplicationIHC

IF2.311

SpeciesMouse

PMID29344222
Twist promotes reprogramming of glucose metabolism in breast cancer cells through PI3K/AKT and p53 signaling pathways

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID26342198
Tumor-secreted GRP78 facilitates the migration of macrophages into tumors by promoting cytoskeleton remodeling

JournalCellular Signalling

ApplicationWB

IF3.388

SpeciesMouse

PMID30959099
Croton tiglium essential oil compounds have anti-proliferative and pro-apoptotic effects in A549 lung cancer cell lines

JournalPLoS One

ApplicationWB

IF2.74

SpeciesHuman

PMID32357169