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FAK (phospho-Y397) polyclonal antibody

货号: BS4617

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4617

Product Name :FAK (phospho-Y397) polyclonal antibody

Swiss-Prot :Q05397

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FAK (phospho-Y397) polyclonal antibody detects endogenous levels of FAK protein only when phosphorylated at Tyr397.

BiowMW :~ 125 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Tumor Angiogenesis,Contribution of ECM and Cytoskeletal Factors to EMT,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2; FAK; FAK1

Immunogen :Synthetic phosphopeptide derived from human FAK around the phosphorylation site of Serine 397.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of FAK (phospho-Y397) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-FAK (Y397) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

PDB-1 from Potentilla discolor Bunge suppresses lung cancer cell migration and invasion via FAK/Src and MAPK signaling pathways

JournalMedicinal Chemistry Research

ApplicationWB

IF1.783

SpeciesHuman

PMID
GSNOR deficiency promotes tumor growth via FAK1 S-nitrosylation

JournalCell Reports

ApplicationIHC

IF9.995

SpeciesMouse

PMID36656716