产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

eIF4B (phospho-S422) polyclonal antibody （KU）

货号: BS4311

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4311

Product Name :eIF4B (phospho-S422) polyclonal antibody （KU）

Swiss-Prot :P23588

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-eIF4B (S422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B

Immunogen :Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-eIF4B (S422) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-eIF4B (S422) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.