Product Name :HSP90B (phospho-S254) polyclonal antibody（KU）
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:1000~1:5000
IHC: 1:50~1:200
IF: 1:50~1:200
Background :HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90alpha, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90beta, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC epsilon to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :HSP90B (phospho-S254) polyclonal antibody detects endogenous levels of HSP90B protein when phosphorylated at Ser254.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics,Hypoxia Signaling,
Alternative Name :Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84; HSP90AB1; HSP90B; HSPC2; HSPCB
Immunogen :Synthetic phosphopeptide derived from human HSP90B around the phosphorylation site of Serine 254.
Modification :Phosphorylation
