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GSK3α (phospho-S21) polyclonal antibody（KU）

货号: BS4082

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS4082

Product Name :GSK3α (phospho-S21) polyclonal antibody（KU）

Swiss-Prot :P49840

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Glycogen synthase kinase-3 α and β (GSK-3α, β) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3β activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser 9 of GSK-3β and inactivates both forms

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-GSK3α (S21) polyclonal antibody detects endogenous levels of GSK3α protein only when phosphorylated at Ser21 .

BiowMW :~ 51 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral elF4 and p70 S6 Kinase,Regulation of Microtubule Dynamics,

Alternative Name :Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

Immunogen :Synthetic phosphopeptide derived from human GSK3α around the phosphorylation site of Serine 21.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-GSK3α (S21) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with UV Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate Lane4:PC12 cell lysate treated with H2O2

Immunohistochemistry (IHC) analyzes of p-GSK3a (S21) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.