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FoxO3A (P247) polyclonal antibody（KU）

货号: BS3574

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3574

Product Name :FoxO3A (P247) polyclonal antibody（KU）

Swiss-Prot :O43524

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Background :FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FoxO3A (P247) polyclonal antibody detects endogenous levels of FoxO3A protein.

BiowMW :~ 80-90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,

Alternative Name :Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

Immunogen :Synthetic peptide, corresponding to amino acids 221-270 of Human FoxO3A.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of FoxO3A (P247) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(30ug) Lane2:SP2/0 whole cell lysate(30ug) Lane3:C6 whole cell lysate(30ug)

Immunohistochemistry (IHC) analyzes of FoxO3A (P247) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunofluorescence analysis of EC9706 cells using FoxO3A (P247) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of EC9706 cell lysates using FoxO3A (P247) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using FoxO3A (P247) pAb.

FOXC2 promotes colorectal cancer proliferation through inhibition of FOXO3a and activation of MAPK and AKT signaling pathways

JournalCancer Letters

ApplicationWB

IF7.36

SpeciesHuman

PMID25069037
Probucol and atorvastatin in combination protect rat brains in MCAO model: Upregulating Peroxiredoxin2, Foxo3a and Nrf2 expression

JournalNeuroscience Letters

ApplicationWB,IHC

IF3.046

SpeciesRat

PMID
SIRT2-mediated FOXO3a deacetylation drives its nuclear translocation triggering FasL-induced cell apoptosis during renal ischemia reperfusion

JournalApoptosis

ApplicationIF,IHC,WB

IF4.677

SpeciesRat

PMID
Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes

JournalJournal Of Lipid Research

ApplicationWB

IF4.483

SpeciesMouse

PMID26113535
Ketogenic Metabolism Inhibits Histone Deacetylase (HDAC) and Reduces Oxidative Stress After Spinal Cord Injury in Rats

JournalNeuroscience

ApplicationWB

IF3.244

SpeciesRat

PMID29024787