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FoxO3A (P247) polyclonal antibody（KU）

货号: BS3574

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3574

Product Name :FoxO3A (P247) polyclonal antibody（KU）

Swiss-Prot :O43524

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Background :FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FoxO3A (P247) polyclonal antibody detects endogenous levels of FoxO3A protein.

BiowMW :~ 80-90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,

Alternative Name :Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

Immunogen :Synthetic peptide, corresponding to amino acids 221-270 of Human FoxO3A.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of FoxO3A (P247) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(30ug) Lane2:SP2/0 whole cell lysate(30ug) Lane3:C6 whole cell lysate(30ug)

Immunohistochemistry (IHC) analyzes of FoxO3A (P247) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunofluorescence analysis of EC9706 cells using FoxO3A (P247) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of EC9706 cell lysates using FoxO3A (P247) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using FoxO3A (P247) pAb.

Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis

JournalChinese Medical Journal

ApplicationWB

IF2.628

SpeciesHuman

PMID26481742
Role of the AMPK signaling pathway in early brain injury after subarachnoid hemorrhage in rats

JournalActa Neurochirurgica

ApplicationWB

IF1.817

SpeciesRat

PMID25697836
Expression of PH Domain Leucine-rich Repeat Protein Phosphatase, Forkhead Homeobox Type O 3a and RAD51, and their Relationships with Clinicopathologic Features and Prognosis in Ovarian Serous Adenocarcinoma

JournalChinese Medical Journal

ApplicationIHC

IF1.555

SpeciesHuman

PMID28139510
GL-V9, a new synthetic flavonoid derivative, ameliorates DSS-induced colitis against oxidative stress by up-regulating Trx-1 expression via activation of AMPK/FOXO3a pathway

JournalOncotarget

ApplicationWB,IF

IF0

SpeciesMouse

PMID26327408
LZ-101, a novel derivative of danofloxacin, induces mitochondrial apoptosis by stabilizing FOXO3a via blocking autophagy flux in NSCLC cells

JournalCell Death & Disease

ApplicationWB,IHC,IF

IF5.959

SpeciesHuman

PMID31217472