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AKR1B1 (K275) polyclonal antibody（KU）

货号: BS3067

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS3067

Product Name :AKR1B1 (K275) polyclonal antibody（KU）

Swiss-Prot :P15121

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Aldose reductase (also designated AKR1B1, ALDR1, ALR2 or AR) is member of the monomeric NADPH-dependent aldoketoreductase family. Aldose reductase, which has a molecular mass of 36 kDa, catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AKR1B1 (K275) polyclonal antibody detects endogenous levels of AKR1B1 protein.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Aldose reductase; ldehyde reductase; Aldo-keto reductase family 1 member B1; AKR1B1; ALDR1

Immunogen :Synthetic peptide, corresponding to amino acids 240-290 of Human AKR1B1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AKR1B1/Aldose Reductase (K275) pAb at 1:1000 dilution Lane1:A549 whole cell lysate(10ug) Lane2:HEK293T whole cell lysate(10ug) Lane3:Hela whole cell lysate(10ug) Lane4:The Embryo tissue lysate of Mouse(40ug) Lane5:The Kidney tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of AKR1B1 (K275) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing screated staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of AKR1B1 (K275) pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing screated staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.