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AChRα1 (E217) polyclonal antibody（KU）

货号: BS2850

价格：￥ 660

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2850

Product Name :AChRα1 (E217) polyclonal antibody（KU）

Swiss-Prot :P02708

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Nicotinic acetylcholine receptors(AChRs) are found at the postsynaptic membrane of the neuromuscular junctionand bind acetylcholine molecules, allowing ions to move through thepore. Glutamate receptors are found in the postsynaptic membrane of cellsin the central nervous system. AChRα1, also known as ACHRD, CHRNA, CMS2A, FCCMS, SCCMS or CHRNA1, is a 482 amino acid multi-pass membrane protein that exists as two alternatively spliced isoforms, which are expressed in different tissues. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AChRα1 (E217) polyclonal antibody detects endogenous levels of AChRα1 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Acetylcholine receptor subunit alpha; ACHRA; CHNRA; CHRNA1; ACHRD; CMS2A; FCCMS, SCCMS; Acra

Immunogen :Synthetic peptide, corresponding to amino acids 181-230 of Human AChRα1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AChRα1 (E217) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of AChRα1 (E217) pAb bin paraffin-embedded human liver carcinoma tissue at 1:50,showing membrnae and cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.