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AIF-M1 (Y85) polyclonal antibody（KU）

货号: BS2407

价格：￥ 660

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS2407

Product Name :AIF-M1 (Y85) polyclonal antibody（KU）

Swiss-Prot :O95831

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase 9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AIF-M1 (Y85) polyclonal antibody detects endogenous levels of AIF-M1 protein.

BiowMW :~ 67 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Apoptosis-inducing factor 1, mitochondrial; Programmed cell death protein 8; AIFM1; AIF; PDCD8

Immunogen :Synthetic peptide, corresponding to amino acids 61-110 of Human AIF-M1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of AIF-M1 (Y85) polyclonal antibody at 1:500 dilution Lane1:The liver tissue lysate of Mouse(39ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of AIF-M1 (Y85) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of AIF-M1 (Y85) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Down‐regulation of Notch receptor signaling pathway induces caspase‐dependent and caspase‐independent apoptosis in lung squamous cell carcinoma cells

JournalApmis

ApplicationWB

IF2.225

SpeciesHuman

PMID22583356