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ICAD (Q177) polyclonal antibody

货号: BS2229

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS2229

Product Name :ICAD (Q177) polyclonal antibody

Swiss-Prot :O00273

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a 112 kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85 kDa apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the 45 kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The 70 kDa nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ICAD (Q177) polyclonal antibody detects endogenous levels of ICAD protein.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD; DFFA; DFF1; DFF45; H13

Immunogen :Synthetic peptide, corresponding to amino acids 151-200 of Human ICAD.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IDCAD (Q177) polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:COS-7 whole cell lysate(40ug) Lane5:SGC7901 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ICAD (Q177) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through Fas- and mitochondria-mediated pathways

JournalMolecular Carcinogenesis

ApplicationWB

IF4.784

SpeciesHuman

PMID22821714