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CYP27A1 (E131) polyclonal antibody（KU）

货号: BS2192

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2192

Product Name :CYP27A1 (E131) polyclonal antibody（KU）

Swiss-Prot :Q02318

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways.[1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3β-hydroxy-5-cholestenoic acid.It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CYP27A1 (E131) polyclonal antibody detects endogenous levels of CYP27A1 protein

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Sterol 26-hydroxylase; mitochondrial; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase; CYP27A1; CYP27

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human CYP27A1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CYP27A1 (E131) pAb at 1:500 dilution Lane1:LO2 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of CYP27A1 (E131) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Effects of chronic dexamethasone exposure on bile acid metabolism and cecal epithelia function in goats

JournalDomestic Animal Endocrinology

ApplicationWB

IF2.302

SpeciesGoat

PMID29803110
Betaine supplementation alleviates corticosterone-induced hepatic cholesterol accumulation through epigenetic modulation of HMGCR and CYP7A1 genes in laying hens

JournalPOULTRY SCIENCE

ApplicationWB

IF4.4

SpeciesChicken

PMID38232620