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14-3-3 ε (D238) polyclonal antibody（KU）

货号: BS2177

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS2177

Product Name :14-3-3 ε (D238) polyclonal antibody（KU）

Swiss-Prot :P62258

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :14-3-3 ε (D238) polyclonal antibody detects endogenous levels of 14-3-3 ε protein.

BiowMW :~ 29 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :14-3-3 protein epsilon; 14-3-3E; YWHAE; 1433E

Immunogen :Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 ε.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of 14-3-3 ε (D238) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of 14-3-3 ε (D238) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of 14-3-3 ε (D238) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.