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Cytokeratin 8 (V67) polyclonal antibody（KU）

货号: BS1211

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1211

Product Name :Cytokeratin 8 (V67) polyclonal antibody（KU）

Swiss-Prot :P05787

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cytokeratin 8 (V67) polyclonal antibody detects endogenous levels of Cytokeratin 8 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,

Alternative Name :Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; Short name=K8; Type-II keratin Kb8; KRT8; CYK8

Immunogen :Synthetic peptide, corresponding to amino acids 41-90 of Human Cytokeratin 8.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cytokeratin 8 (V67) polyclonal antibody at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:NIH-3T3 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Cytokeratin 8 (V67) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.