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Adducin α/β (K720) polyclonal antibody

货号: BS1005

价格：￥ 660

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS1005

Product Name :Adducin α/β (K720) polyclonal antibody

Swiss-Prot :P35611/P35612

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:50~1:200

Background :Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Adducin α/β (K720) polyclonal antibody detects endogenous levels of Adducin α and Adducin β protein.

BiowMW :~ 120 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Alpha-adducin; Erythrocyte adducin subunit alpha; ADD1; ADDA; α-Adducin; Adducin1; Adducin-1; α Adducin; Adducin-α; Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β

Immunogen :Synthetic peptide, corresponding to amino acids 700-750 of Human Adducin α.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Adducin α/β (K720) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(30ug) Lane2:C6 whole cell lysate(30ug) Lane3:CT26 whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Adducin α/β (K720) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.