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AChE polyclonal antibody（KU）

货号: AP0691

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :AP0691

Product Name :AChE polyclonal antibody（KU）

Swiss-Prot :P22303

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Acetylcholinesterase (AChE) hydrolyzes acetylcholine at synaptic junctions. Alternative mRNA splicing gives rise to three forms of AChE. The T form, also known as the asymmetric form, is soluble and is present in synapses. The H form is also known as the globular form and is present on the outer surfaces of cell membranes. The R form is not known to be a functional species. AChE globular form subunits are GPI-anchored to cell membranes and asymmetric subunits are anchored to basal lamina components by a collagen tail. The catalytic sununits of AChE are oligomers composed of disulfide-linked homodimers. The loss of AChE from cholinergic and noncholinergic neurons in the brain is seen in patients with Alzheimer’s disease. However, AChE activity is increased around amyloid plaques, which may be due to a disturbance in calcium homeostasis involving the opening of L-type voltage-dependent calcium channels.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 117% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ACHE polyclonal antibody detects endogenous levels of ACHE protein.

BiowMW :~ 70-85 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Acetylcholinesterase; AChE; ACHE; ACES

Immunogen :Synthetic peptide corresponding to residues in Human AChE.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of AChE pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:The Blood tissue lysate of Mouse(40ug) Lane4:The Blood tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of AChE pAb bin paraffin-embedded human lung carcinoma tissue at 1:50,showing membrnae and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of AChE pAb bin paraffin-embedded human rectum carcinoma tissue at 1:50,showing membrnae and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Akebia saponin D attenuates ibotenic acid-induced cognitive deficits and pro-apoptotic response in rats: Involvement of MAPK signal pathway

JournalPharmacology Biochemistry And Behavior

ApplicationWB

IF2.519

SpeciesRat

PMID
Activation of mTOR signaling mediates the increased expression of AChE in high glucose condition: in vitro and in vivo evidences

JournalMolecular Neurobiology

ApplicationWB

IF4.5

SpeciesRat,Mouse

PMID26374551
Alleviating Effects of Bushen-Yizhi Formula on Ibotenic Acid-Induced Cholinergic Impairments in Rat

JournalRejuvenation Research

ApplicationWB

IF3.811

SpeciesRat

PMID25482164