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Mouse Proinsulin (1A3-5) monoclonal antibody

货号: MB67284

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB67284

Product Name :Mouse Proinsulin (1A3-5) monoclonal antibody

Swiss-Prot :P01325; P01326

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF/ICC IP

Application_all :WB: 1:1000~1:2000 IHC: 1:200~1:1000 IF/ICC: 1:200 IP: 1:125~1:250

Background :Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Product :PBS, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse Proinsulin (1A3-5) monoclonal antibody detects endogenous levels of proinsulin protein.

BiowMW :~ 12 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Insulin precursor ; Proinsulin

Immunogen :A synthetic peptide of Mouse proinsulin C-peptide and B chain.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Mouse Proinsulin (1A3-5) monoclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate,transfected with pCDNA3.1 (non-reduced) Lane2:HEK293T whole cell lysate,transfected with human proinsulin-myc-flag (non-reduced) Lane3:HEK293T whole cell lysate,transfected with pCDNA3.1 (reduced) Lane4:HEK293T whole cell lysate,transfected with human proinsulin-myc-flag (reduced) Lane5:INS1 whole cell lysate (non-reduced) Lane6:INS1 whole cell lysate (reduced) Lane7:Min6 whole cell lysate (non-reduced) Lane8:Min6 whole cell lysate (reduced)

Immunohistochemical analysis of Mouse Proinsulin (1A3-5) staining in Mouse pancreas tissue section.

Immunofluorescent analysis of Mouse Proinsulin (1A3-5) staining in Min6 cells.

Western blot (WB) analysis of Mouse Proinsulin (1A3-5) monoclonal antibody at 1:125 dilution Lane1:INS1 whole cell lysate Lane2:Immunoprecipitation of IgG Lane3:Immunoprecipitation of Mouse Proinsulin (1A3-5) monoclonal antibody Lane4:Immunoprecipitation of Proinsulin (161-1) antibody