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Human Proinsulin (3L10) monoclonal antibody

货号: MB67283

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB67283

Product Name :Human Proinsulin (3L10) monoclonal antibody

Swiss-Prot :P01308

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF/ICC IP

Application_all :WB: 1:1000~1:2000 IHC: 1:500~1:1000 IF/ICC: 1:200 IP: 1:250

Background :Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Product :PBS, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Human Proinsulin (3L10) monoclonal antibody detects endogenous levels of proinsulin protein.

BiowMW :~ 12 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Insulin precursor ; Proinsulin

Immunogen :A synthetic peptide of human proinsulin C-peptide and B chain.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Human Proinsulin (3L10) monoclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate,transfected with pCDNA3.1 (non-reduced) Lane2:HEK293T whole cell lysate,transfected with human proinsulin-myc-flag (non-reduced) Lane3:HEK293T whole cell lysate,transfected with pCDNA3.1 (reduced) Lane4:HEK293T whole cell lysate,transfected with human proinsulin-myc-flag (reduced) Lane5:INS1 whole cell lysate (non-reduced) Lane6:INS1 whole cell lysate (reduced) Lane7:Min6 whole cell lysate (non-reduced) Lane8:Min6 whole cell lysate (reduced)

Western blot (WB) analysis of Human Proinsulin (3L10) monoclonal antibody at 1:1000 dilution Lane1:Supernatant secreted by human insulinoma cells (non-reduced) Lane2:Supernatant secreted by human insulinoma cells (reduced)

Immunohistochemical analysis of Human Proinsulin (3L10) staining in Mouse pancreas and human pancreas tissue section.

Western blot (WB) analysis of Human Proinsulin (3L10) monoclonal antibody at 1:250 dilution Lane1:HEK293T whole cell lysate,transfected with human proinsulin-myc-flag Lane2:Immunoprecipitation of IgG Lane3:Immunoprecipitation of Human Proinsulin (1B24) monoclonal antibody Lane4:Immunoprecipitation of Human Proinsulin (3L10) monoclonal antibody Lane5:Immunoprecipitation of Proinsulin (160-2) antibody