产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Rb polyclonal antibody

货号: BZ16670

价格：￥ 1140

货期：现货（1-2个工作日）

25ug 50ug 100ug 200ug

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BZ16670

Product Name :Rb polyclonal antibody

Swiss-Prot :P06400

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB,IHC,ICC/IF,IP

Application_all :WB: 1/500-1/1000 IHC: 1/100-1/200 IF/ICC: 1/100-1/500 IP: 1/10-1/50

Background :Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rb polyclonal antibody detects endogenous levels of Rb protein.

BiowMW :~ 110 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RB.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of Rb expression in PC3 (A), Hela (B), NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of Rb staining in human stomach cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of Rb staining in HuvEc cells.

免疫沉淀（IP）