产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

DDX3X polyclonal antibody

货号: BS8482

价格：￥ 1140

货期：现货（1-2个工作日）

25ug 50ug 100ug 200ug

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS8482

Product Name :DDX3X polyclonal antibody

Swiss-Prot :O00571

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC-P, IF/ICC, IP

Application_all :WB,1:500 - 1:1000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :DDX3X polyclonal antibody detects endogenous levels of DDX3X protein.

BiowMW :75KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DDX3X;CAP-Rf;DBX;DDX14;DDX3;HLP2;MRX102

Immunogen :A synthetic peptide of human DDX3X(NP_001347.3).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of various lysates using DDX3X pAb at 1:1000 dilution.

Immunohistochemistry analysis of paraffin-embedded Rat lung using DDX3X pAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX3X pAb at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 300 μg extracts of C6 cells using 3 μg DDX3X antibody. Western blot was performed from the immunoprecipitate using DDX3X antibody at a dilution of 1:3000.