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E-Cadherin polyclonal antibody

货号: BS79669

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79669

Product Name :E-Cadherin polyclonal antibody

Swiss-Prot :P12830

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP, FC

Application_all :WB,1:500 - 1:1000|IHC,1:100 - 1:200|IF/ICC,1:100 - 1:500|IP,1:10 - 1:100|FC,1:100 - 1:200

Background :This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :E-Cadherin polyclonal antibody detects endogenous levels of E-Cadherin protein.

BiowMW :120KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human E-Cadherin.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of E-Cadherin expression in A375 (A), HepG2 (B), mouse brain (C), rat brain (D) whole cell lysates.

Immunohistochemical analysis of E-Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of E-Cadherin staining in PC12 cells.

Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate, using 5ug of Anti-E-Cadherin Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).