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Prdm9 polyclonal antibody

货号: BS78012

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78012

Product Name :Prdm9 polyclonal antibody

Swiss-Prot :Q96EQ9

Host :Rabbit

Reactivity :Mouse, Rat

Applications :IF/ICC

Application_all :IF/ICC,1:50 - 1:200

Background :Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4 and 'Lys-36' (H3K36 of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3 and trimethylated 'Lys-36' (H3K36me3 histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Prdm9 polyclonal antibody detects endogenous levels of Prdm9 protein.

BiowMW :97KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Rc; re; Dsbc; Rcr1; Dsbc1; Meise; repro7; Meisetz; PRDM9-B; BC012016; G1-419-29

Immunogen :Recombinant fusion protein of mouse Prdm9(NP_659058.3).

Conjugate :Unconjugated

Modification :Unmodified

Immunofluorescence analysis of mouse testis using Prdm9 pAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of rat testis using Prdm9 pAb at dilution of 1:100 (40x lens).

免疫沉淀（IP）

免疫组织化学（IHC）