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BTBD1 polyclonal antibody

货号: BS76153

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS76153

Product Name :BTBD1 polyclonal antibody

Swiss-Prot :Q9H0C5

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB FC

Application_all :WB,1:500 - 1:1000|FC,1:10 - 1:50

Background :The C-terminus of the protein encoded by this gene binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BTBD1 polyclonal antibody detects endogenous levels of BTBD1 protein.

BiowMW :55kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :C15orf1; NS5ATP8; BTB/POZ domain-containing protein 1; Hepatitis C virus NS5A-transactivated protein 8; HCV NS5A-transactivated protein 8

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human BTBD1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of BTBD1 expression in 293 (A) whole cell lysates.

Flow cytometric analysis of 293 cells using Anti-BTBD1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.