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NR1I3 polyclonal antibody

货号: BS7155

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7155

Product Name :NR1I3 polyclonal antibody

Swiss-Prot :Q14994

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NR1I3 polyclonal antibody detects endogenous levels of NR1I3 protein.

BiowMW :45KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NR1I3;CAR;CAR1;NR1I3

Immunogen :Recombinant fusion protein of human NR1I3(NP_001070948.1).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of extracts of various cell lines, using NR1I3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of HepG2 cells using NR1I3 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunoprecipitation of NR1I3 in 300 µg extracts from 293T cells transfected with NR1I3 using 3 µg NR1I3 pAb. Western blot analysis was performed using NR1I3 pAb at 1:1000 dilution.