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Tubulin β (4D9) monoclonal antibody

货号: BS1482M

价格：￥

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1482M

Product Name :Tubulin β (4D9) monoclonal antibody

Swiss-Prot :Q13509/P04350/P68371/P07437/Q13885/Q9BUF5

Host :Mouse

Reactivity :Human,Mouse, Rabbit, Frog, Fish, Rat

Applications :WB IHC IF IP

Application_all :WB: 1:5000~20000 IHC: 1:100~500 IF: 1:100~500 IP: 1:100~500

Background :Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Total tubulin β; Tubulin beta

Immunogen :Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Tubulin β (4D9) mAb at 1:5000/1:10000/1:20000 dilution Lane1:L02 whole cell lysate(20ug) Lane2:NIH-3T3 whole cell lysate(20ug) Lane3:PC12 whole cell lysate(20ug) Lane4:The Kidney tissue lysate of Mouse(20ug)

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