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Flag-Tag mouse monoclonal antibody

货号: AP1020M

价格：￥

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP1020M

Product Name :Flag-Tag mouse monoclonal antibody

Host :Mouse

Reactivity :Transfected

Applications :WB IHC IF IP

Application_all :WB: 1:20000~1:100000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Background :Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Flag-Tag mAb detects over-expressed or recombinant proteins containing the Flag epitope tag.

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK

Immunogen :Synthetic peptide Flag-tag.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Flag-Tag mAb at 1:100000 dillution Lane1:HEK293F whole cell lysate ,untransfected Lane2:HEK293F whole cell lysate , transfected (M-Flag) Lane3:HEK293F whole cell lysate , transfected (N-Flag) Lane4:HEK293F whole cell lysate , transfected (C-Flag)

Immunofluorescent analysis of Flag-Tag mAb staining in transfected HEK293F cells.

Steroid hormone ouabain cooperates with Na+,K+-ATPase to improve innate immunity against bacterial infections through a non-classic training mechanism regulated by mrna reprogramming

JournalActa Pharmaceutica Sinica B

ApplicationWB

IF14.6

SpeciesHuman

PMID