Product Name :DDDDK-Tag (3B9) monoclonal antibody
Application_all :WB: 1:5000~1:10000
Background :Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.
Specificity :DDDDK-tag Mouse antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
Figure1 :Western blot (WB) analysis of DYKDDDDK-Tag(3B9)monoclonal antibody at 1:5000 dilution
Lane1:HEK293T whole cell lysate,transfected with pcDNA3.1-DDDDK-p62-HA.
Lane2:HEK293T whole cell lysate
Lane3:SK-OVCAR3 whole cell lysate
Lane4:H9C2 whole cell lysate
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Immunogen :Synthetic peptide DDDDK-tag.
Modification :Unmodification
