Product Name :GFP-tag polyclonal antibody
Application_all :WB 1:1000 - 1:5000
Background :Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :GFP-tag polyclonal antibody detects over-expressed or recombinant proteins containing the GFP epitope tag.
Figure1 :Western blot analysis of GFP fusion protein using GFP-Tag antibody at different dilution.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Green fluorescent protein
Immunogen :Recombinant protein of full length GFP.
Modification :Unmodification