Product Name :Tubulin β (4D9) monoclonal antibody
Reactivity :H,M,R(not applicable to tissue)
Swiss-Prot :Q13509/P04350/P68371/P07437/Q13885/Q9BUF5
Application_all :WB: 1:5000~20000
IHC: 1:100~500
IF: 1:100~500
Background :Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein.
Figure1 :Western blot (WB) analysis of Tubulin β (4D9) mAb at 1:5000/1:10000 dilution
Lane1:C6 whole cell lysate(20ug)
Lane2:CT26 whole cell lysate(20ug)
Lane3:MCF-7 whole cell lysate(20ug)
Lane4:A549 whole cell lysate(20ug)
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Total tubulin β; Tubulin beta
Immunogen :Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β.
Modification :Unmodification
