中国 EN 400-660-9091

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Tubulin β polyclonal antibody

货号: AP0064
价格 400
货期 现货(1-2个工作日)
25ug 50ug 100ug 200ug
No. :AP0064
Product Name :Tubulin β polyclonal antibody
Host :Rabbit
Reactivity :Human,Mouse,Rat,Odontobutis obscurus(not applicable to some tissue)
Applications :WB IP IF
Swiss-Prot :N/A
Application_all :WB:1:5000~1:20000 IP: 1:100~500 IF: 1:500~1000
Background :Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Tubulin β polyclonal antibody pAb detects endogenous levels of Tubulin β protein.
Figure1 :Western blot (WB) analysis of Tubulin β pAb at 1:5000/1:10000/1:20000/1:40000 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:MG63 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug) Lane6:The Brain tissue lysate of Rat(40ug) Lane7:The Brain tissue lysate of Mouse(40ug)
Figure2 :Western blot (WB) analysis of Tubulin β pAb at 1:5000 dilution Lane1:A549 whole cell lysate(20ug) Lane2:PC12 whole cell lysate(20ug) Lane3:BV2 whole cell lysate(20ug) Lane4:The Kidney tissue lysate of Mouse(20ug) Lane5:The Prostate tissue lysate of Rat(20ug)
Figure3 :Immunofluorescence analysis of RAW264.7 cells using Tubulin β pAb at dilution of 1:1000 (40x lens).
Figure4 :Immunoprecipitation of MCF-7 cell lysates using Tubulin β pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3).Lane 1 is 30% input. The western blot was probed using Tubulin β mAb.
BiowMW :~ 55 kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4
Immunogen :Recombinant full length Human Tubulin β.
Conjugate :Unconjugated
Modification :Unmodified
  • Exosomal miR-146a Contributes to the Enhanced Therapeutic Efficacy of Interleukin-1β-Primed Mesenchymal Stem Cells Against Sepsis
    JournalStem Cells
    ApplicationWB
    IF6.277
    SpeciesHuman
  • PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-γ to increase surface FasL
    JournalApoptosis
    ApplicationWB
    IF4.021
    SpeciesRat
  • Effect of low-level mechanical vibration on osteogenesis and osseointegration of porous titanium implants in the repair of long bone defects
    JournalScientific Reports
    ApplicationWB
    IF3.998
    SpeciesRabbit
  • Phytic acid attenuates inflammatory responses and the levels of NF-κB and p-ERK in MPTP-induced Parkinson’s disease model of mice
    JournalNeuroscience Letters
    ApplicationWB
    IF2.274
    SpeciesMouse
  • Impairment of mesenchymal stem cells derived from oral leukoplakia
    JournalInternational Journal of Clinical and Experimental Pathology
    ApplicationWB
    IF0.252
    SpeciesHuman

关注我们

400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com