Product Name :DDDDK-tag polyclonal antibody
Application_all :WB: 1:2000~1:5000
IP: 1:50~1:200
IF: 1:50~1:200
Background :Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :DDDDK-tag polyclonal antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
Figure1 :Western blot (WB) analysis of DDDDK-tag pAb at 1:5000 dillution
Lane1:HEK293T whole cell lysate
Lane2:HEK293T whole cell lysate,transfected with pcDNA3.1-DDDDK-p62-HA.
Figure2 :IF image of AP0007 stained HEK293T cells, transfected with pcDNA3.1-HA-DDDDK-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody DDDDK-tag pAb #AP0007(1:200) and the antibody HA-tag (4G3) mAb #AP0005M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) wasGoat anti-Rabbit IgG (H+L) -FITC#BS10950 and the secondary antibody (Red) was Goat anti-Mouse IgG (H+L)-TRITC#BS11502. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
Figure3 :Immunoprecipitation of 293F cell transfected with pcDNA3.4-TfR-DDDDK lysates using DDDDK-Tag pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using DDDDK-Tag pAb #AP0007.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Immunogen :Synthetic peptide DDDDK-tag
Modification :Unmodification
