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CD146 polyclonal antibody（KU）

货号: NCP0270P

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :NCP0270P

Product Name :CD146 polyclonal antibody（KU）

Swiss-Prot :P43121

Host :Rabbit

Reactivity :Human

Applications :WB IP IF

Application_all :WB: 1:2000~1:5000 IF 1:50~1:200 IP 1:50 - 1:200

Background :Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CD146 polyclonal antibody detects endogenous levels of CD146 protein.

BiowMW :~110 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146

Immunogen :Recombinant protein of human CD146.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of CD146 polyclonal antibody at 1:2000 dilution Lane1:L02 cell membrane lysate(20ug) Lane2:A549 cell membrane lysate(20ug) Lane3:MCF-7 cell membrane lysate(20ug)

Immunofluorescence analysis of L02 cells using CD146 pAb at dilution of 1:200.

Western blot (WB) analysis of AIF polyclonal antibody at 1:5000 dilution Blocking buffer: 5% BSA in TBST. Lane1:HEK293T whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(30ug) Lane3:H9C2 whole cell lysate(30ug) Lane4:CT-26 whole cell lysate(30ug) Lane5:The kidney tissue lysate of Rat(30ug) Lane6:The stomach tissue lysate of Mouse(30ug)