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AMPKβ1 (1A7) monoclonal antibody

货号: MB0188

价格：￥ ——

货期：现货（1-2个工作日）

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No. :MB0188

Product Name :AMPKβ1 (1A7) monoclonal antibody

Swiss-Prot :Q9Y478

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey

Applications :WB ICC IHC IP

Application_all :WB: 1:1000 IP: 1:50~200 ICC: 1:200 IHC: 1:800

Background :AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA. It also regulates cholesterol synthesis via phosphorylation and in-activation of hydroxymethylglutaryl-CoA reductase.Two isoforms of AMPKβ1 have been found.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of AMPKβ1 and does not cross-react with related proteins.

BiowMW :Predicted band size:38KDa Observed band size:38KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hypoxia Signaling,AMPK Signaling Pathway,MTOR signaling,

Alternative Name :5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1; AMPK; AMPK β1

Immunogen :Purified recombinant human AMPKβ1 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of AMPKβ1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPKβ1 mouse mAb (1:1000 diluted).

Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPKβ1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPKβ1 mouse mAb (dilution 1:100).

Immunoprecipitation analysis of Hela cell lysates using AMPKβ1 mouse mAb.