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EGFR (3F12) monoclonal antibody

货号: MB0182

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB0182

Product Name :EGFR (3F12) monoclonal antibody

Swiss-Prot :P00533

Host :Mouse

Reactivity :Human,Monkey

Applications :WB,ICC/IF,IP

Application_all :WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Background :The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of EGFR and does not cross-react with related proteins.

BiowMW :~ 175 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,

Alternative Name :Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR

Immunogen :Purified recombinant human EGFR protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of EGFR in A549 and COS7 lysates using EGFR antibody.

Immunocytochemistry analysis of EGFR in HeLa using EGFR antibody.

Immunoprecipitation analysis of EGFR in Hela lysates using EGFR antibody.

In vitro and in vivo antitumor effect of gefitinib nanoparticles on human lung cancer

JournalDrug Delivery

ApplicationIHC

IF6.419

SpeciesMouse

PMID28961023