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FEN1 (7H8) monoclonal antibody

货号: MB0179

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0179

Product Name :FEN1 (7H8) monoclonal antibody

Swiss-Prot :P39748

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB ICC

Application_all :WB: 1:1000 ICC: 1:1000~500

Background :DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5' flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of FEN1 and does not cross-react with related proteins.

BiowMW :Predicted band size:42KDa Observed band size:42KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1; FEN1; RAD2

Immunogen :Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).

免疫沉淀（IP）

免疫组织化学（IHC）