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E2F-1 (4G8) monoclonal antibody

货号: MB0176

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB0176

Product Name :E2F-1 (4G8) monoclonal antibody

Swiss-Prot :Q01094

Host :Mouse

Reactivity :Human,Rat

Applications :WB IF IP

Application_all :WB: 1:500 IP: 1:50~200 IF: 1:50~200

Background :The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of E2F-1 and does not cross-react with related proteins.

BiowMW :Predicted band size:47KDa Observed band size:70KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,

Alternative Name :Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1; RBBP3

Immunogen :Purified recombinant human E2F-1 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).

Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).

Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.

Downregulation of CDK-8 inhibits colon cancer hepatic metastasis by regulating Wnt/β-catenin pathway

JournalBiomedicine & Pharmacotherapy

ApplicationWB

IF6.529

SpeciesMouse

PMID26349978