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eIF2α (2E4) monoclonal antibody

货号: MB0174

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0174

Product Name :eIF2α (2E4) monoclonal antibody

Swiss-Prot :P05198

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:1000 IF: 1:50~200

Background :eIF2α, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2α controls the rate of tRNA translation. When eIF2α is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2α is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of eIF2α and does not cross-react with related proteins.

BiowMW :Predicted band size:38KDa Observed band size:38KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral Regulation of elF2,

Alternative Name :Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2A; EIF2S1

Immunogen :Purified recombinant human eIF2α protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of eIF2α in K562,COS7,3T3, C2C12,MCF-7,C6 and Hela cell lysates using eIF2α mouse mAb (1:1000 diluted).

Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-eIF2α mouse mAb (dilution 1:200).