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HDAC4 (N-terminus) monoclonal antibody

货号: MB0041

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB0041

Product Name :HDAC4 (N-terminus) monoclonal antibody

Swiss-Prot :P56524

Host :Mouse

Reactivity :Human

Applications :WB ICC

Application_all :WB: 1:1000 ICC: 1:50~200

Background :In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBPassociated factor), HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of HDAC4 (N-terminus) and does not cross-react with related proteins

BiowMW :Predicted band size:120KDa Observed band size:130KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Notch Signaling,Nuclear Receptor Signaling,Cell Cycle Control G1 S Checkpoint,Epigenetic Writers and Erasers of Histone H3,Epigenetic Writers and Erasers of Histones H2A H2B and H4,

Alternative Name :Histone deacetylase 4; HD4; HDAC4; KIAA0288

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human HDAC4.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of HDAC4 in Jurkat, Hela&K562 cell lysates using HDAC4 antibody (1:1000 diluted) .

Immunocytochemistry of HeLa cells using anti- HDAC4 (N-terminus) antibody diluted 1:150.