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DLAT monoclonal antibody

货号: MB0031

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0031

Product Name :DLAT monoclonal antibody

Swiss-Prot :P10515

Host :Mouse

Reactivity :Human,Mouse

Applications :WB ICC IP

Application_all :WB: 1:1000 ICC: 1:300 IP: 1:50~1:200

Background :This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

Product :Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of Pyruvate Dehydrogenase E2 and does not cross-react with related proteins

BiowMW :Predicted band size:69KDa Observed band size:69KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :AMPK Signaling Pathway,

Alternative Name :70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoamide S Acetyltransferase; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; DLAT; DLTA; E2; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; mitochondrial; ODP2; PBC; PDC E2; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2; Pyruvate dehydrogenase complex E2 subunit; DLTA; PDCE2; PDC-E2; Pyruvate Dehydrogenase E2

Immunogen :Recombinant full length Human DLAT.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of DLAT in Jurkat, A549, U251, F9, LNCAP&Hela cell lysates using DLAT antibody (1:1000 diluted) .

Immunocytochemistry stain of Hela using DLAT antibody (1:300).