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FADD polyclonal antibody

货号: CAS7512

价格：￥ ——

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :CAS7512

Product Name :FADD polyclonal antibody

Swiss-Prot :Q13158

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:2000~1:5000

Background :Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8. FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent and may be related to its regulatory role in embryonic development and cell cycle progression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FADD polyclonal antibody detects endogenous levels of FADD protein.

BiowMW :~ 30 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Toll-like Receptor signaling,Death Receptor Signaling,

Alternative Name :FAS-associated death domain protein; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADD; FADD; GIG3

Immunogen :Recombinant protein of human FADD.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of FADD polyclonal antibody at 1:5000 dilution Lane1:Pancl whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)

Functional Role of Fatty Acyl-Coenzyme A Synthetase in the Transmembrane Movement and Activation of Exogenous Long-chain Fatty Acids: AMINO ACID RESIDUES WITHIN THE ATP/AMP SIGNATURE MOTIF OFESCHERICHIA COLI FadD ARE REQUIRED FOR ENZYME ACTIVITY AND FATTY

JournalJournal Of Biological Chemistry

ApplicationWB

IF5.157

SpeciesEscherichia coli

PMID12034706
Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through Fas- and mitochondria-mediated pathways

JournalMolecular Carcinogenesis

ApplicationWB

IF4.784

SpeciesHuman

PMID22821714
Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways

JournalJournal Of Applied Toxicology

ApplicationWB

IF3.065

SpeciesHuman

PMID22334414