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BID polyclonal antibody（KU）

货号: CAS75116

价格：￥ 780

货期：现货（1-2个工作日）

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No. :CAS75116

Product Name :BID polyclonal antibody（KU）

Swiss-Prot :P55957

Host :Rabbit

Reactivity :Human

Applications :WB IP IHC

Application_all :WB: 1:2000~1:5000 IP:1:2000~1:8000 IHC:1:50~1:100

Background :Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax. Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane. Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation. Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BID polyclonal antibody detects endogenous levels of BID protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :BH3-interacting domain death agonist; p22 BID; BID; p15 BID; p13 BID; p11 BID

Immunogen :Recombinant protein of human BID.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of BID polyclonal antibody at 1:5000 dilution Lane1:Jurkat whole cell lysate(40ug) Lane2:THP-1 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug)

Immunoprecipitation - BID Polyclonal Antibody

Immunohistochemistry of paraffin-embedded human colon carcinoma using BID antibody at dilution of 1:50.