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Rock-1 polyclonal antibody（KU）

货号: CAS75111

价格：￥ 780

货期：现货（1-2个工作日）

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No. :CAS75111

Product Name :Rock-1 polyclonal antibody（KU）

Swiss-Prot :Q13464

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP

Application_all :WB: 1:5000~1:10000 IP:1:2000~1:8000

Background :ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains.The C-terminus of ROCK negatively regulates its kinase activity. Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB)activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rock-1 polyclonal antibody detects endogenous levels of Rock-1 protein.

BiowMW :~ 180kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Microtubule Dynamics,

Alternative Name :Rho-associated protein kinase 1;Renal carcinoma antigen NY-REN-35;Rho-associated, coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;P160rock;ROCK1

Immunogen :Recombinant protein of human Rock-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Rock-1 polyclonal antibody at 1:5000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)

Immunoprecipitation - Rock-1 Polyclonal Antibody

Alleviation of the doxorubicin-induced nephrotoxicity by fasudil in vivo and in vitro

JournalJournal Of Pharmacological Sciences

ApplicationWB

IF2.835

SpeciesRat

PMID33357780
Opticin Ameliorates Hypoxia-Induced Retinal Angiogenesis by Suppression of Integrin α2-I Domain–Collagen Complex Formation and RhoA/ROCK1 Signaling

JournalInvestigative Ophthalmology & Visual Science

ApplicationWB

IF4.799

SpeciesZebrafish

PMID35006271