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CLIC1 polyclonal antibody

货号: BS8158

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8158

Product Name :CLIC1 polyclonal antibody

Swiss-Prot :O00299

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF/ICC IP

Application_all :WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 1:50 - 1:200

Background :Chloride intracellular channel 1 (CLIC1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. CLIC1 is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as a anionselective channel. CLIC1 forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring. Insulin concentration also plays a role in CLIC1 regulation, and the hormone may cause a subnuclear relocalization of CLIC1. CLIC1 is associated with macrophage activation; a downregulation of CLIC1 function prevents TNFα release induced by β-Amyloid protein (A-β) stimulation. This suggests a role for CLIC1 in several neurodegenerative processes, such as Alzheimer’s disease, a syndrome characterized by an accumulation of A-β.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CLIC1 polyclonal antibody detects endogenous levels of CLIC1 protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Chloride intracellular channel protein 1; Chloride channel ABP; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC; CLIC1; G6; NCC27

Immunogen :Recombinant Protein corresponding to a sequence within amino acids 42-241 of human CLIC1(NP_001279.2).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of lysates from MCF7 cells, using CLIC1 pAb at 1:1000 dilution.

Western blot analysis of lysates from wild type(WT) and CLIC1 knockdown (KD) HeLa cells, using CLIC1 pAb at 1:1000 dilution.

Immunofluorescence analysis of K-562 cells using CLIC1 pAb at dilution of 1:200 (40x lens).

Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg CLIC1 pAb.